CELL LINES
Contributor Information
- Name Heather Mckinnon ; Justin Bower ; Daniel Croft
- Institute Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
- Tool name: MDA-MB-231 D3H2LN-Luc TetOn-3G cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MDA-MB-231 D3H2LN-Luc
- Organism: Human
- Tissue: Breast
- Cancer type: Breast cancer
- Disease: Cancer
- Growth properties: Adherent cell line
- Model: Reporter
- Conditional: Yes
- Conditional description: When a gene of interest is introduced under the control of the tetracycline (Tet)-regulated transactivator Tet-On 3G expression can be induced by administration of doxycycline. Luciferase expression is non-conditional.
- Description: This cell line contains a tetracyline inducible promoter construct ready for cloning in a gene of interest. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Unbekandt et al., 2014 introduced their genes of interest, the kinases MRCK?, ROCK1 and ROCK2 under the tetracycline inducible promoter, to study the impact of their expression and inhibition on migration of the cells. Useful for the study of breast cancer cell cytoskeleton reorganisation, cell migration and the development of therapies that inhibit the ability of primary tumours to metastasize. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
- Research area: Cancer; Cell biology; Drug development; Genetics
- Production details: Generated an MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G
- Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
- • A novel small-molecule MRCK inhibitor blocks cancer cell invasion.
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