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Contributor Information

  • Name Heather Mckinnon ; Justin Bower ; Daniel Croft
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: MDA-MB-231 D3H2LN-Luc TetOn-3G cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MDA-MB-231 D3H2LN-Luc
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Triple negative breast cancer (TNBC)
  • Disease: Cancer
  • Description: This cell line contains a tetracyline inducible promoter construct ready for cloning in a gene of interest. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Unbekandt et al., 2014 introduced their genes of interest, the kinases MRCK?, ROCK1 and ROCK2 under the tetracycline inducible promoter, to study the impact of their expression and inhibition on migration of the cells. Useful for the study of breast cancer cell cytoskeleton reorganisation, cell migration and the development of therapies that inhibit the ability of primary tumours to metastasize. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
  • Research area: Cancer ; Cell Structure and Motility ; Drug Discovery & Development ; Genetic Studies Tools

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1


  • Available on request


  •   Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
  •   A novel small-molecule MRCK inhibitor blocks cancer cell invasion.