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Contributor Information

  • Name Heather Mckinnon ; Justin Bower ; Daniel Croft
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: MDA-MB-231 TetOn-3G ROCK1 cell line
  • Alternate names: Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I, p16 ROCK-1, p16ROCK
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MDA-MB-231 D3H2LN-Luc cell line
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Growth properties: Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
  • Model: Transgenic
  • Conditional: Yes
  • Conditional description: MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK1. Therefore ROCK1 expression is induced in the presence of doxycycline.
  • Description: This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK1. ROCK1 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK1 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Useful for the study of breast cancer cell cytoskeleton reorganisation and cell migration. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
  • Research area: Cancer; Cell biology; Drug development; Genetics
  • Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.

  • For Research Use Only

Target Details

  • Target: ROCK1

Application Details


  • Format: Frozen
  • Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
  •   A novel small-molecule MRCK inhibitor blocks cancer cell invasion.