The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA.
| Institute |
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| Vlaams Instituut voor Biotechnologie (VIB) |
| SKU: | 154153 |
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| Product description: | The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA. The human embryonic kidney cells at first seemed recalcitrant to transformation. After m… |
| Conditional: | No |
| Production details: | HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA |
| Cellosaurus ID: | CVCL_0045 |
| Parental cell line: | Human embryonic kidney cells |
| Cat. #: | 154153 |
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| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Kidney |
| Model: | Immortalised Line |
| Format: | Frozen |
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| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | EMEM (EBSS) + 2mM Glutamine + 1% Non-Essential Amino Acids (NEAA) + 10% FCS |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Lin et al. 2014. Nat Commun. 5:4767. PMID: 25182477. Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Stillman et al. 1985. Mol Cell Biol. 5(8):2051-60. PMID: 3018548. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells. |
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