CELL LINES
Contributor Information
- Institute Vlaams Instituut voor Biotechnologie (VIB)
Tool Details
- Tool name: HEK 293 cell line (genotyped)
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: Human embryonic kidney cells
- Organism: Human
- Tissue: Kidney
- Model: Immortalised Line
- Conditional: No
- Description: The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA. The human embryonic kidney cells at first seemed recalcitrant to transformation. After many attempts, cell growth took off only several months after the isolation of a single transformed clone. A 4-kbp adenoviral genome fragment is known to have integrated in chromosome 19 and encodes for the E1A/E1B proteins, which interfere with the cell cycle control pathways and counteract apoptosis. Cytogenetic analysis established that the 293 line is pseudotriploid. These HEK 293 have been whole-genome re-sequenced at passage number 35. The article by Lin et al al, allows user friendly visualisation of the genome data. The genome structure composition is in steady state when standard cell culturing conditions are used.
- Research area: Cancer
- Production details: HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA
- Cellosaurus ID: CVCL_0045
- For Research Use Only
Target Details
Application Details
- Application notes: These HEK 293 have been whole-genome re-sequenced at passage number 35. The article by Lin et al al, allows user friendly visualisation of the genome data. The genome structure composition is in steady state when standard cell culturing conditions are used.
Handling
- Format: Frozen
- Growth medium: EMEM (EBSS) + 2mM Glutamine + 1% Non-Essential Amino Acids (NEAA) + 10% FCS
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
Related Tools
References
- • Lin et al. 2014. Nat Commun. 5:4767. PMID: 25182477.
- • Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
- • Stillman et al. 1985. Mol Cell Biol. 5(8):2051-60. PMID: 3018548.
- • Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
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