2C4-Gamma2A cell line
- Name George Stark ; Ian Kerr
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool name: 2C4-Gamma2A cell line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: 2C4 cell line (a derivative of HT 1080 cell line)
Cancer type: Fibrosarcoma
Description: Mutant gamma2A cells retain a wild-type interferon (IFN)-alpha/beta response (e.g., class I HLA Binding of IFN-gamma to gamma2A cells is normal), but the cells lack detectable JAK2 mRNA and protein and are completely defective in all aspects of the IFN-gamma response. More specifically, they are defective in IFN-gamma inducible activation of the JAK/STAT pathway, gene expression, and an antiviral state with respect to encephalomyocarditis (EMC) virus. In all cases, JAK2 (but not JAK1 or Tyk2 restores a wild-type phenotype. The gamma2A mutant cells lack inducible phosphorylation of the IFN-gamma receptor 1 (R1) subunit, JAK1, and STAT1 and IFN-gamma-inducible cell surface expression of class I and II HLAs. In each case, the wild-type IFN-gamma response is restored in gamma2A/JAK2 transfectants (Kohlhuber et al., 1997).
Research area: Cell Signaling & Signal Transduction ; Gene Expression ; Immunology
- For Research Use Only
- • Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
- • A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
- • Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
- • Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.