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Contributor Information

  • Name George Stark ; Ian Kerr
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: 2C4 cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HT 1080
  • Organism: Human
  • Cancer type: Fibrosarcoma
  • Disease: Cancer
  • Description: The 2C4 cell line is a clonal derivative of the human fibrosarcoma cell line HT 1080. The 2C4 cell line clone expresses the simple cell-surface marker CD2 (normally expressed only on T cells) under the control of the 9-27 gene promoter. The 9-27 gene promoter is inducible by interferon gamma.
  • Research area: Cancer ; Cell Signaling & Signal Transduction ; Immunology

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 10% heat-inactivated FBS + 400ug G418 per ml (As described by Kohlhuber et al., 1997)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
  •   A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
  •   Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
  •   Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.