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Contributor Information

  • Name Aloysius Klingelhutz
  • Institute The University of Iowa

Tool Details

  • Tool name: 16E6/E7 HCK cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Human ectocervix cells
  • Organism: Human
  • Tissue: Cervix
  • Growth properties: E6/E7-immortalized cells generally showed an increase in telomere length as they were passaged in culture, with some later passage lines having telomeres that were similar to or longer than the earliest-passage precrisis cells examined.
  • Application: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.
  • Description: Expresses cervical epithelia cell markers; responds to inflammatory agents; supports growth of cervical pathogens. Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.
  • Research area: Cancer
  • Production details: The HPV16 E6/E7 open reading frames were cloned into a murine-based retroviral vector, LXSN, which contains the neomycin resistance gene. The constructs were transfected into the packaging celline PA317, and recombinant retroviruses in the supernatant were collected. Early-passage cx cells were infected at a high titer with the different retroviral constructs, plated at various densities, selected in G418, and then passaged as pools or ring cloned. Eight ring clones were isolated for each group. Cells were passaged 1:5 when confluent.

  • For Research Use Only

Target Details

  • Target: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies

Application Details

  • Application: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.

Handling

  • Format: Frozen
  • Growth medium: GIBCO keratinocyte serum-free media. G418 selection.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Klingelhutz et al. 1994. Mol Cell Biol. 14(2):961-9. PMID: 8289836.
  • •  Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.