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Contributor Information

  • Name Aloysius Klingelhutz
  • Institute The University of Iowa

Tool Details

  • Tool name: 16E6/E7-HSK cell line
  • Tool type: Cell Lines
  • Tool sub-type: Primary
  • Parental cell line: Keratinocytes isolated from punch biopsies of the skin from the buttocks
  • Organism: Human
  • Tissue: Skin
  • Disease: Cancer
  • Growth properties: Upregulation of telomerase and greatly extended lifespan (greater than 5 times the lifespan of untransduced keratinocytes) with no apparent crisis or slow down in growth. Despite upregulation of telomerase, E6/E7 transduced keratinocytes exhibited significant telomere shortening with passaging in culture
  • Conditional: No
  • Description: Expresses skin cell markers; proinflammatory. Tissue is adult skin cells.
  • Research area: Cancer
  • Production details: Cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418

  • For Research Use Only

Target Details

  • Target: Used as an immortal adult human skin keratinocyte line for pathogenesis and inflammation studies

Application Details


  • Format: Frozen
  • Growth medium: G418 selection.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498.
  •   Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA compon