CELL LINES
Contributor Information
- Name Aloysius Klingelhutz
- Institute The University of Iowa
Tool Details
- Tool name: 16E6/E7-HSK cell line
- Tool type: Cell Lines
- Tool sub-type: Primary
- Parental cell line: Keratinocytes isolated from punch biopsies of the skin from the buttocks
- Organism: Human
- Tissue: Skin
- Disease: Cancer
- Growth properties: Upregulation of telomerase and greatly extended lifespan (greater than 5 times the lifespan of untransduced keratinocytes) with no apparent crisis or slow down in growth. Despite upregulation of telomerase, E6/E7 transduced keratinocytes exhibited significant telomere shortening with passaging in culture
- Conditional: No
- Description: Expresses skin cell markers; proinflammatory. Tissue is adult skin cells.
- Research area: Cancer
- Production details: Cells were transduced with HPV-16 E6/E7 LXSN replication defective retrovirus and selected in G418
- For Research Use Only
Target Details
- Target: Used as an immortal adult human skin keratinocyte line for pathogenesis and inflammation studies
Application Details
Handling
- Format: Frozen
- Growth medium: G418 selection.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
References
- • Gourronc et al. 2010. Exp Dermatol. 19(3):279-88. PMID: 19558498.
- • Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA compon
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