ZR-75-1 [VIII-21] cell line
- Name Lambert Dorssers
- Institute Erasmus University Medical Center (Erasmus MC)
Tool name: ZR-75-1 [VIII-21] cell line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: ZR-75-1
Cancer type: Mammary ductal carcinoma; Estrogen independent
Model: Cancer Model
Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Retroviral insertion mutagenesis was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method the main genes conferring estrogen independence in human breast cancer cells where identified. Genes located in the immediate proximity of the retroviral integration site were characterised. Out of 15 candidate breast cancer antigen resistance (BCAR) genes, seven (AKT1, AKT2, BCAR1, BCAR3, EGFR2, GRB7 and TRERF1/BCAR2) were shown to directly underline estrogen independence. Cell line with established retrovirus target responsible for estrogen independence: BCAR1
This cell line is part of a panel of 71 cell lines (Cat No 154549-154619) plus the parental (Cat No 154547).
These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity.
Research area: Cancer; Drug Discovery & Development
Production details: ZR-75-1 cells were infected with amphotropic, defective murine retrovirus and plated in medium containing 1uM of 4-hydroxy-tamoxifen. Within 5 weeks after the start of selection proliferating colonies were individually picked and expanded to stable cell lines
- For Research Use Only
- • van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453.
- • Dorssers et al. 1993. Mol Endocrinol. 7(7):870-8. PMID: 8413311.