CELL LINES
Contributor Information
- Name Lambert Dorssers
- Institute Erasmus University Medical Center (Erasmus MC)
Tool Details
- Tool name: ZR-75-1 HygroR [ZH3D7] cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: ZR-75-1
- Organism: Human
- Tissue: Breast
- Cancer type: Gynaecologic cancer
- Disease: Cancer
- Model: Cancer Model
- Description: Hygromycin-B-resistant variant of ZR-75-1 human breast-cancer cells. These cells were used as acceptor cells for somatic cell fusion experiments
- Research area: Cancer
- Production details: ZR-75-1 cells were transfected with the expression construct PGK-Hyg Br which confers hygromycin B resistance using cationic liposome. Stable transfectants were selected with 60ug/ml of hygromycin B in RBCS medium supplemented with 1nM estradiol.
- Additional notes: These cells were used as acceptor cells for somatic cell fusion experiments
- For Research Use Only
Target Details
Application Details
- Application notes: The cell line is resistant to Geneticin, which may be included in the culture medium to ensure that the expression vector is retained by the cells.
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS) and 1 nM 17?-estradiol
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Dorssers et al. 1997. Int J Cancer. 72(4):700-5. PMID: 9259413.
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