CELL LINES
Contributor Information
- Name Lambert Dorssers
- Institute Erasmus University Medical Center (Erasmus MC)
Tool Details
- Tool name: ZR-75-1 PDGFRB [ZR-PDGFRB (4147)] cell line
- Alternate names: Platelet Derived Growth Factor Receptor Beta; PDGFR1; CD14B
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: ZR-75-1
- Organism: Human
- Tissue: Breast
- Cancer type: Gynaecologic cancer
- Disease: Cancer
- Model: Cancer Model
- Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in advanced disease cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the parental (Cat No 154547).
- Research area: Cancer; Drug development
- Production details: Retroviral particles containing PDGFRB cDNA were produced by transient transfection of Phoenix-Ampho packaging cells using FuGENE6. ZR-75-1 cells were incubated with viral particles in the presence of 4ug/ml polybrene and 1nmol/L estradiol. Three days after transfection cells were cultured in medium supplemented with G418 and resistant colonies were expanded.
- For Research Use Only
Target Details
- Target: PDGFRB
Application Details
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
References
- • van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.
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