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Contributor Information

  • Name Lambert Dorssers
  • Institute Erasmus University Medical Center (Erasmus MC)

Tool Details

  • Tool name: MCF7A AKT1 [MCF7-AKT1] cell line
  • Alternate names: AKT Serine/Threonine Kinase 1; Protein Kinase B; PKB; RAC
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7A
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Gynaecologic cancer
  • Disease: Cancer
  • Model: Cancer Model
  • Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many disease cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in MCF7A human breast cancer cells. This cell line is part of a panel of 4 cell lines (Cat No 154636-154638, 154643) which have been transfected with these genes, plus the parental (Cat No 154546) These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
  • Research area: Cancer; Drug development
  • Production details: Full length AKT1 cDNA was introduced in the estrogen-dependent MCF7A cell line by transfection with lipofectamine

  • For Research Use Only

Target Details

  • Target: AKT1

Application Details

  • Application notes: This cell line is also resistant to Geneticin which may be included in the culture to maintain the expression plasmid


  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.