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Contributor Information

  • Name Lambert Dorssers
  • Institute Erasmus University Medical Center (Erasmus MC)

Tool Details

  • Tool name: ZH3D7:BCAR1 [D4E6] Fusion cell line
  • Alternate names: Breast Cancer Anti-Estrogen Resistance 1; CASS1; P13Cas; CRKAS
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: ZH3D7
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Mammary ductal carcinoma, estrogen independent
  • Disease: Cancer
  • Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Somatic cell fusion was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method revealed that tamoxifen resistance co-segregated with only 1 or 2 of the integration loci present in the tamoxifen-resistant donor cell line, designated the first breast cancer anti-estrogen resistance locus (BCAR1) This cell line is part of a panel of 3 somatic hybrids (Cat No 154639-154641) plus the parental (Cat 154620). This cell line is a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression and therapy resistance.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: R/BCS medium containing estradiol and Geneticin (G418)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.