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Contributor Information

  • Institute Erasmus University Medical Center (Erasmus MC)

Tool Details

  • Tool name: ZR-75-1 estrogen independent breast cancer cell line panel (2)
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: ZR-75-1
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Mammary ductal carcinoma, estrogen independent
  • Disease: Cancer
  • Description: Endocrine therapy of breast cancer has been applied widely and proven to be effective. However, in many instances endocrine treatments ultimately fail due to the development of an estrogen-independent therapy resistant phenotype. To elucidate the molecular mechanism underlying this endocrine therapy failure, the laboratory of Lambert Dorssers applied different genetic screens to identify the main genes conferring estrogen independence. Out of 15 candidate BCAR genes, several including BCAR1, BCAR3, AKT1, AKT2, BCAR4, GRB7, EGFR, HRAS, PDGFRA, PDGFRB and RAF1 were shown to directly underlie estrogen independence by transfection into the ZR-75-1 breast cancer cell line resulting in a panel of 16 cell lines (Cat No 154621-154635, 154642) These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Godinho et al. 2011. J Cell Physiol. 226(7):1741-9. PMID: 21506106.
  •   van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.
  •   van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453.
  •   Meijer et al. 2006. Mol Cancer Res. 4(6):379-86. PMID: 16778085.
  •   Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.
  •   van Agthoven et al. 1998. EMBO J. 17(10):2799-808. PMID: 9582273.
  •   van Agthoven et al. 1992. Cancer Res. 52(18):5082-8. PMID: 1516065.