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Contributor Information

  • Institute Erasmus University Medical Center (Erasmus MC)

Tool Details

  • Tool name: MCF7A estrogen independent breast cancer cell line panel
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7A
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Mammary adenocarcinoma, estrogen-dependent
  • Disease: Cancer
  • Model: Cancer Model
  • Conditional: No
  • Description: Endocrine therapy of breast cancer has been applied widely and proven to be effective. However, in many instances endocrine treatments ultimately fail due to the development of an estrogen-independent therapy resistant phenotype. To elucidate the molecular mechanism underlying this endocrine therapy failure, the laboratory of Lambert Dorssers applied different genetic screens to identify the main genes conferring estrogen independence. Out of 15 candidate BCAR genes, several including BCAR3, BCAR4 and AKT1 were shown to directly underlie estrogen independence to MCF7A breast cancer cells. These genes were transfected into the MCF7A breast cancer cell line resulting in a panel of 4 cell lines (Cat No 154636-154638, 154643). These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
  • Research area: Cancer ; Drug Discovery & Development
  • Production details: Full length cDNA of the relevant gene was introduced in the estrogen-dependant MCF7A cell line by transfection with lipofectamine

  • For Research Use Only

Target Details

  • Target: BCAR3, BCAR4, AKT1

Application Details

  • Application notes: The cell lines are also resistant to Geneticin which may be included in the culture to maintain the expression plasmid

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS)
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Godinho et al. 2011. J Cell Physiol. 226(7):1741-9. PMID: 21506106.
  •   van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.
  •   van Agthoven et al. 1998. EMBO J. 17(10):2799-808. PMID: 9582273.