CELL LINES
Contributor Information
- Name Daniel Peet
- Institute The University of Adelaide
Tool Details
- Tool name: SIRMu-1 Cell Line
- Tool type: Cell Lines
- Organism: Rat
- Tissue: Eye
- Disease: Retinal studies into Müller cell function. Müller cells are a major type of glia cell in the retina of the eye
- Growth properties: large and flat with a "ghost-like" appearance.
- Model: Immortalised Line
- Description: Both the cellular morphology and overall transcriptome of the SIRMu-1?cells are more similar to primary rat MCs than the commonly used rMC-1?cells
- Research area: Drug development; Neurobiology
- Production details: A monoclonal line dervied from two sequential rounds of single cell cloning by serial dilution. No Virus or any pathogen. Not GMO. The cells have not been transformed with any agents. The cells can be passaged 1:3 to 1:5 every 2-4 days. Estimated population doubling times with cultured in 10% and 20% FBS are 36 and 30 hours respectively. The cells can take time to recover after thawing from a frozen stock. After thawing, it is quite normal to have a lot of dead and floating cells. For a vial which contains cells from a third of a confluent T175 flask, it is recommended to thaw into a T25 flask. It can then take up to a week for confluency. Before a complete recovery, the doubling time might be longer than normal.
- Cellosaurus ID: CVCL_UW38
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: MEM*, 20% FBS, 25mM glucose (final concentration), 2mM L-glutamine. *Minimum Essential Medium (+Earle's Salts, -L glutamine, Gibco #11090). Note: The cells also grow fine in 10% FBS and/or 5mM glucose.
- Shipping conditions: Dry ice
Documentation
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