USP9x-deficient HCT116 (with or without p53) cell line
- Name Fred Bunz
- Institute Johns Hopkins University
Tool name: USP9x-deficient HCT116 (with or without p53) cell line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: HCT 116
Description: This cell line presents an opportunity to study the effect of loss of apoptotic control on colorectal cancer cells. Study of deubiquitinase Ups9X could act as a leap towards understanding apoptotic regulation and potentially lead to new drug targets for cancer.In this technology, the gene USP9X is knocked out of two the HCT116 cell line. The gene knockout has been performed by rAAV-mediated homologous recombination. The technology also consists of a double knockout line without USP9X and p53, a tumor suppressor gene.
Research area: Cancer; Drug development
- For Research Use Only
- • Harris et al. 2012. Cancer Biol Ther. 13(13):1319-24. PMID: 22895071.