R-3327-AT-1 cell line
- Name John Isaacs
- Institute Johns Hopkins University
Tool name: R-3327-AT-1 cell line
Tool type: Cell Lines
Cancer type: Genitourinary cancer
Model: Cancer Model
Description: AT-1 originates from the spontaneous tumor (R3327) of the prostate identified by W.F. Dunning in a 22 month-old inbred Copenhagen male rat in 1961. R3327 has been maintained by continuous serial passage in rats for many years. In the course of the passage of the H subline in intact (i.e. not castrated) male rats, random tumor progression occurred due to the genetic instability of the tumor in an occasional rat, giving rise to fast growing anaplastic tumors within a single passage. AT-1 was the first of such anaplastic tumors to arise. Following subcutaneous transplantation in syngeneic male rats, AT-1 forms solid sheets of malignant cells with no indication of glandular function. These tumors are androgen-independent and grow continuously with a doubling time of approximately 2.5 days. AT-1 exhibits a low metastatic ability (<5% of rats inoculated sub-cutaneously develop distant metastases). The AT-1 subline can be used to study the effects of various types of cancer therapies on prostate cells.
Research area: Cancer; Drug development
Cellosaurus ID: CVCL_3568
- For Research Use Only
- • Isaacs et al. 1988. Prostate. 13(2):165-88. PMID: 3174494.
- • Isaacs et al. 1986. Prostate. 9(3):261-81. PMID: 3774632.