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Contributor Information

  • Name John Isaacs
  • Institute Johns Hopkins University

Tool Details

  • Tool name: R-3327-AT-1 cell line
  • Tool type: Cell Lines
  • Parental cell line:
  • Cancer type: Prostate cancer
  • Disease: Cancer
  • Description: AT-1 originates from the spontaneous tumor (R3327) of the prostate identified by W.F. Dunning in a 22 month-old inbred Copenhagen male rat in 1961. R3327 has been maintained by continuous serial passage in rats for many years. In the course of the passage of the H subline in intact (i.e. not castrated) male rats, random tumor progression occurred due to the genetic instability of the tumor in an occasional rat, giving rise to fast growing anaplastic tumors within a single passage. AT-1 was the first of such anaplastic tumors to arise. Following subcutaneous transplantation in syngeneic male rats, AT-1 forms solid sheets of malignant cells with no indication of glandular function. These tumors are androgen-independent and grow continuously with a doubling time of approximately 2.5 days. AT-1 exhibits a low metastatic ability (<5% of rats inoculated sub-cutaneously develop distant metastases). The AT-1 subline can be used to study the effects of various types of cancer therapies on prostate cells.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium:
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Isaacs et al. 1988. Prostate. 13(2):165-88. PMID: 3174494.
  •   Isaacs et al. 1986. Prostate. 9(3):261-81. PMID: 3774632.