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Contributor Information

  • Name Mark Donowitz
  • Institute Johns Hopkins University

Tool Details

  • Tool name: NHE-2 expressing PS120 cell line
  • Tool type: Cell Lines
  • Parental cell line: CCL39
  • Disease: Na+ absorption and diarrhea
  • Description: An isoform of the Na+/H+ exchanger, NHE-2, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:• NHE-2 can be used to study Na+ absorption and function for treatment of diarrhea when NHE-3 is inhibited by cAMP/cholera toxin
  • Research area: Cell Signaling & Signal Transduction ; Drug Discovery & Development

  • For Research Use Only

Target Details

  • Target: Na+/H+ exchanger

Application Details

Handling

  • Format: Frozen
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References