CELL LINES
Contributor Information
- Name Caroline Hill
- Institute The Francis Crick Institute
Tool Details
- Tool name: MDA-MB-231 CAGA12-Luc/TK Renilla cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MDA-MB-231 cell line
- Organism: Human
- Tissue: Breast
- Cancer type: Breast cancer
- Disease: Cancer
- Model: Reporter
- Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
- Description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
- Research area: Cancer; Cell signaling and signal transduction; Developmental biology
- Production details: Made by transfecting the breast cancer cell line MDA-MB-231 with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Clonal
- For Research Use Only
Target Details
- Target: Activin and transforming growth factor ÄÂ? (TGF-ÄÂ?Â)ÄÂ? signalling
Application Details
Handling
- Format: Frozen
- Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Gr
nroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.
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