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Contributor Information

  • Name Caroline Hill
  • Institute The Francis Crick Institute

Tool Details

  • Tool name: MDA-MB-231 CAGA12-Luc/TK Renilla cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MDA-MB-231 cell line
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Reporter
  • Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
  • Description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
  • Research area: Cancer ; Cell Signaling & Signal Transduction ; Developmental Biology
  • Production details: Made by transfecting the breast cancer cell line MDA-MB-231 with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Clonal

  • For Research Use Only

Target Details

  • Target: Activin and transforming growth factor β (TGF-β) signalling

Application Details

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.