CELL LINES
Contributor Information
- Name Caroline Hill
- Institute The Francis Crick Institute
Tool Details
- Tool name: HEK293T CAGA12-Luc/TK Renilla cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HEK293T cell line
- Organism: Human
- Tissue: Kidney
- Model: Reporter
- Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
- Description: The human embryo kidney cell line HEK293T modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
- Research area: Cancer; Cell signaling and signal transduction; Developmental biology
- Production details: Made by transfecting the human embryo kidney cell line HEK293T with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Pool
- Additional notes: Unpublished
- For Research Use Only
Target Details
- Target: Activin and transforming growth factor ÄÂ? (TGF-ÄÂ?Â)ÄÂ? signalling
Application Details
Handling
- Format: Frozen
- Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Unpublished
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