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Contributor Information

  • Name Caroline Hill
  • Institute The Francis Crick Institute

Tool Details

  • Tool name: MDA-MB-231 BRE-Luc/TK Renilla cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MDA-MB-231 cell line
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Reporter
  • Conditional description: Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
  • Description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor ? (TGF-?) superfamily member.
  • Research area: Cancer; Cell signaling and signal transduction; Developmental biology
  • Production details: Made by transfecting the breast cancer cell line MDA-MB-31 with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance. Clonal

  • For Research Use Only

Target Details

  • Target: Bone morphogenetic protein (BMP) signalling

Application Details

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.