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Contributor Information

  • Name Caroline Hill
  • Institute The Francis Crick Institute

Tool Details

  • Tool name: HEK293T BRE-Luc/TK Renilla cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HEK293T cell line
  • Organism: Human
  • Tissue: Kidney
  • Description: The human embryo kidney cell line HEK293T modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor ? (TGF-?) superfamily member.
  • Research area: Cancer ; Cell Signaling & Signal Transduction ; Developmental Biology

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Unpublished