CELL LINES
Contributor Information
- Name Caroline Hill
- Institute The Francis Crick Institute
Tool Details
- Tool name: HEK293T BRE-Luc/TK Renilla cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HEK293T cell line
- Organism: Human
- Tissue: Kidney
- Model: Reporter
- Conditional description: Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
- Description: The human embryo kidney cell line HEK293T modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor ? (TGF-?) superfamily member.
- Research area: Cancer; Cell signaling and signal transduction; Developmental biology
- Production details: Made by transfecting the human embryo kidney cell line HEK293T with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance. Pool
- Additional notes: Unpublished
- For Research Use Only
Target Details
- Target: Bone morphogenetic protein (BMP) signalling
Application Details
Handling
- Format: Frozen
- Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Unpublished
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