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Contributor Information

  • Name Ana Maria Zubiaga ; Iraia Garcia Santisteban
  • Institute University of the Basque Country (EHU)

Tool Details

  • Tool name: U2OS E2F7 KO cell line
  • Alternate names: U-2 OS
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: U2OS
  • Organism: Human
  • Tissue: Bone
  • Cancer type: Osteosarcoma cell line
  • Disease: Cancer
  • Description: E2F7 is a transcription factor that participates in various processes such as cell cycle regulation and DNA damage response by mediating transcriptional repression of target genes involved in G1/S regulation as well as in DNA replication and repair. E2F7 behaves as a tumour suppressor by inhibiting DNA repair activity of tumour cells upon chemotherapy treatment. Loss of E2F7 by CRISPR knockout confers increased resistance to chemotherapy in BRCA2-deficient cells.
  • Research area: Cancer ; Cell Cycle ; Other
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Mitxelena et al. 2018. Nucleic Acids Res. 46(9):4546-4559. PMID: 29590434.