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Contributor Information

  • Name Aintzane Asumendi ; Aintzane Apraiz ; Dolores Boyano
  • Institute University of the Basque Country (EHU)

Tool Details

  • Tool name: CCRF-CEM-R5 Cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: CCRF-CEM
  • Organism: Human
  • Tissue: Bone Marrow
  • Cancer type: Cancer, Acute Lymphoblastic Leukemia
  • Disease: Cancer, Acute Lymphoblastic Leukemia
  • Growth properties: Cell grow in suspension
  • Model: Tumour line
  • Conditional: No
  • Description: 4-HPR is a synthetic retinoid with potent pro-apoptotic activity against several types of cancer, including leukemia cells.
  • Research area: Cancer ; Drug Discovery & Development ; Other
  • Production details: CCRF-CEM-R5 cell line, resistant to up to 5 mM of 4-HPR, was obtained by gradual selection. Parental CCRF-CEM cells were continuously exposed to increasing 4-HPR concentrations, starting from 0.5 mM.

  • For Research Use Only

Target Details

  • Target: Relevant disease (Acute Lymphoblastic Leukemia/ALL); Drug (synthetic retinoid 4-HPR: fenretinide or N-(4-hydroxyphenyl)retinamide)

Application Details

Handling

  • Format: Frozen
  • Growth medium: Cells should be grown in RPMI 1640 with 2mM L-glutamine supplemented with 10% heat inactivated FBS. Keep at 37°C in a humidified incubator containing 5% CO2. In order to preserve their resistant intermitent exposures to 5mM 4-HPR is recomended. 4-HPR removal from R5 cell culture for up to 8 passages or 3 weeks has not shown any decrease on drug resistance although it does affect endogenous sphingolipid profile (specified in the reference paper).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Apraiz et al. 2011. BMC Cancer. 11:477. PMID: 22061047.