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Contributor Information

  • Name Abey Bandara
  • Institute Virginia Tech

Tool Details

  • Tool name: HEK293?NDUFS2 Cell Line
  • Alternate names: HEK293?NDUFS2
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HEK293
  • Organism: Human
  • Disease: Leighs; Mitochondrial disease; cardiomyopathy; encephalomyopathy
  • Description: The HEK293?NDUFS2 cell line expresses a CRISPR/Cas9 mutated NDUFS2, shown to significantly alter the function of complex I (CI) of the mitochondrial electron transport chain. This NDUFS2 mutant cell line displays disruptions to NDUFS2 that mimic isolated complex I deficiency and can be used as a powerful model of several types of mitochondrial disease. Specific disruptions in the NDUFS2 gene have been reported to present clinically as the following disease conditions: -Leigh syndrome -Cardiomyopathy -Encephalomyopathy -Miscellaneous: basal ganglia and brainstem lesions, seizures, hypotonia, neonatal hypotonia, amaurosis, nystagmus, dysmorphic features, epilepsy and signs of brainstem involvement, and lactic acidosis.
  • Research area: Apoptosis and Programmed Cell Death ; Drug Discovery & Development ; Metabolism
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin at 37°C temperature and 5-6% CO2.
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes

Documentation

  • Available on request

References

  •   Bandara et al. 2021. Mitochondrion. 58:160-168. PMID: 33744462.