Product Image

Contributor Information

  • Name Sarah-Maria Fendt
  • Institute Vlaams Instituut voor Biotechnologie (VIB)

Tool Details

  • Tool name: MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)
  • Alternate names: transforming protein p21, h-Ras, H-Ras, HRAS
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF10A
  • Tissue: Breast
  • Disease: Cancer
  • Model: Cancer Model
  • Description: MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity. This is a control line for the MCF10 H-Ras12 cell line from the same group at the VIB as it has the same pLA expression vector stably expressed but lacking the HRAS gene.
  • Research area: Cancer
  • Additional notes: This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.

  • For Research Use Only

Target Details

  • Target: HRas

Application Details

  • Application notes: This cell line is a negative control for the cell line overexpressing HRas. It was made using the same pLA vector but lacking the HRAS gene.

Handling

  • Format: Frozen
  • Growth medium: DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml-1), 1% streptomycin (50 ?g ml-1), 0.5 ?g ml-1 hydrocortisone, 100 ng ml-1 cholera toxin, 10 ?g ml-1 insulin and 20 ng ml-1 recombinant human EGF.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

Related Tools

Product Image

ZR-75-1 [XIV-42] cell line

#154612
Product Image

ZR-75-1 EGFR [ZR-HERc 1A)] cell line

#154628
Product Image

Omega-E Packaging Cell Line

#151463

References

  • •  Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.