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Contributor Information

  • Name Sarah-Maria Fendt
  • Institute Vlaams Instituut voor Biotechnologie (VIB)

Tool Details

  • Tool name: MCF10A pLA(empty) (negCTRL for hRas_V12 cell line)
  • Alternate names: transforming protein p21, h-Ras, H-Ras, HRAS
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF10A
  • Tissue: Breast
  • Disease: Cancer
  • Description: MCF10A cells are frequently used to study signalling pathways in breast cancer. They can be grown in 3D culture. The addition of the H-Ras12 simulates breast cancer as 50% of breast cancers display increased H-Ras activity. This is a control line for the MCF10 H-Ras12 cell line from the same group at the VIB as it has the same pLA expression vector stably expressed but lacking the HRAS gene.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM-F12 supplemented with 5% horse serum, 1% penicillin (50 units ml-1), 1% streptomycin (50 ?g ml-1), 0.5 ?g ml-1 hydrocortisone, 100 ng ml-1 cholera toxin, 10 ?g ml-1 insulin and 20 ng ml-1 recombinant human EGF.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Elia et al. 2017. Nat Commun. 8:15267. PMID: 28492237.