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Contributor Information

  • Name Martin Clynes
  • Institute Dublin City University

Tool Details

  • Tool name: DLKP-M cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: DLKP
  • Organism: Human
  • Tissue: Lung
  • Cancer type: Lung carcinoma
  • Disease: Cancer
  • Description: Mesenchymal-like sub-population cloned at NCTCC from DLKP P5, about 5% of DLKP cells show DLKP-M morphology, interconversion with -I, closest to normal chromosome number, fold resistance comp. to DLKP: 0.8 Adr; 0.95 Vcr; 0.7 VP-16. Immunocytochemistry: cytokeratin (all)-; GFAP-vimentin+; neurofilament+; P-gp-; a2- integrin++; 703D4+; 704A1 +; desmin-. More invasive in vitro than DLKP-SQ.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F12, 5% FCS. Split 1:5, medium change 2-3 times weekly. Freezing method: 10% DMSO in FBS
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   O'Sullivan et al. 2014. Tumour Biol. 35(7):6983-97. PMID: 24744142.
  •   Keenan et al. 2012. Exp Cell Res. 318(5):593-602. PMID: 22281030.
  •   McBride et al. 1998. Tumour Biol. 19(2):88-103. PMID: 9486560.