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Contributor Information

  • Name Thorbald Van Hall
  • Institute Leiden University and Leiden University Medical Center; Stichting Oncode Institute

Tool Details

  • Tool name: RMA.Trh4 cells
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: RMA
  • Organism: Mouse
  • Tissue: Lymphatic Tissue
  • Cancer type: Mouse Leukemia
  • Disease: Cancer
  • Description: This cell line provides a model to help to understand T-cell recognition through Trh4 expression. ER-resident ceramide synthase Trh4 is a prototypic example of a neo-antigen selectively presented by cells through various pathways. RMA cells have their TAP chain intact and the Trh4 peptide is only presented on cells with impaired TAP function through the classical MHC I pathway. This cell line over-expresses endogenous Trh4 and provides a key tool to help understand presentation of Trh4 to antigen recognition though other pathways.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Retroviral transduction of the mouse Trh4 gene in an IRES-GFP construct
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Oliveira et al. 2011. Eur J Immunol. 41(11):3114-24. PMID: 21898382.