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Contributor Information

  • Name Michael Tomlinson
  • Institute University of Birmingham

Tool Details

  • Tool name: HEK-ADAM10 CRISPR cell line
  • Alternate names: A Disintegrin And Metalloproteinase domain-containing protein 1, CRISPR-Cas9
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HEK 293T
  • Organism: Human
  • Tissue: Kidney
  • Disease: Cancer, Alzheimer's and inflammatory diseases
  • Description: A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines. CRISPR edited ADAM 10 HEK-293T cells. Generation of CRISPR/Cas9-knockout cell line: A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5’-CACCGATACCTCTCATATTTACAC-3’ and 5’-AAACGTGTAAATATGAGAGGTATC-3’)
  • Research area: Cancer ; Neurobiology ; Other
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796. This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium:
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
  •   Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.