HEK-ADAM10 CRISPR cell line
- Name Michael Tomlinson
- Institute University of Birmingham
Tool name: HEK-ADAM10 CRISPR cell line
Alternate names: A Disintegrin And Metalloproteinase domain-containing protein 1, CRISPR-Cas9
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: HEK 293T
Disease: Cancer; Alzheimer's; Inflammatory diseases
Description: A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer?Âs and inflammatory diseases. ADAM10 is a ?Âmolecular scissor?Â that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines.
CRISPR edited ADAM 10 HEK-293T cells.
Generation of CRISPR/Cas9-knockout cell line:
A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5?Â-CACCGATACCTCTCATATTTACAC-3?Â and 5?Â-AAACGTGTAAATATGAGAGGTATC-3?Â)
Research area: Cancer; Neurobiology
- For Research Use Only
- • Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
- • Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.