CELL LINES
Contributor Information
- Name Michael Tomlinson
- Institute University of Birmingham
Tool Details
- Tool name: HEK-ADAM10 CRISPR cell line
- Alternate names: A Disintegrin And Metalloproteinase domain-containing protein 1, CRISPR-Cas9
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HEK 293T
- Organism: Human
- Tissue: Kidney
- Disease: Cancer; Alzheimer's; Inflammatory diseases
- Description: A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer?Âs and inflammatory diseases. ADAM10 is a ?Âmolecular scissor? that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines. CRISPR edited ADAM 10 HEK-293T cells. Generation of CRISPR/Cas9-knockout cell line: A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5?Â-CACCGATACCTCTCATATTTACAC-3? and 5?Â-AAACGTGTAAATATGAGAGGTATC-3?Â)
- Research area: Cancer; Neurobiology
- For Research Use Only
Target Details
- Target: ADAM10
Application Details
Handling
- Format: Frozen
- Growth medium: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
- • Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.
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