Product Image

Contributor Information

  • Name Diane Lee
  • Institute University of Surrey

Tool Details

  • Tool name: Bovine Type II Alveolar Epithelial (B2AE) cell line
  • Tool type: Cell Lines
  • Tissue: Alveolus
  • Growth properties: Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI)
  • Model: Immortalised Line
  • Description: Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus.
  • Research area: Immunology ; Other
  • Production details: Generated by the lentiviral transduction of Bmi1 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. Proliferation rate as for primary.

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture Âź dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References