CELL LINES
Contributor Information
- Name Diane Lee
- Institute University of Surrey
Tool Details
- Tool name: Bovine Type II Alveolar Epithelial (B2AE) cell line
- Tool type: Cell Lines
- Tissue: Alveolus
- Growth properties: Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI)
- Model: Immortalised Line
- Description: Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus.
- Research area: Immunology ; Other
- Production details: Generated by the lentiviral transduction of Bmi1 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. Proliferation rate as for primary.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture ĂĹş dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2.
- Shipping conditions: Dry ice
Documentation
- Available on request
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