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Contributor Information

  • Name Julian Downward
  • Institute The Francis Crick Institute

Tool Details

  • Tool name: 3LL NRAS KO #86 cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: 3LL aka LL/2 (LLC1) likely origin - please note genetic divergence in relevance information
  • Organism: Mouse
  • Tissue: Lung
  • Cancer type: Lung Adenocarcinoma
  • Disease: Cancer
  • Description: Murine Lewis Lung Adenocarcinoma engineered cell line with NRAS knocked out using CRISPR-Cas9. Please note: The authors have established (by DNA sequencing) that these cells are homozygous for the KRAS-G12C & NRAS-Q61H mutations which is not the case for the parent 3LL ATCC line. It is assumed both mutant NRAS and KRAS in 3LL cells drive RAS pathway activation. By knocking out NRAS a tumourigenic cell line with a stronger dependency on KRAS-G12C was generated. Model cell line for study of the effect of KRAS-G12C inhibitors on oncogenic cells.
  • Research area: Cancer ; Cell Cycle ; Cell Signaling & Signal Transduction
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI+10%FCS+1%PS
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Molina-Arcas et al. 2019. Sci Transl Med. 11(510):. PMID: 31534020.