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Contributor Information

  • Name Carlos Caldas
  • Institute University of Cambridge

Tool Details

  • Tool name: MCF7 ZsGreen-ZNF703 Overexpressing Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Knock-In
  • Conditional: No
  • Description: This is a derivative of the MCF7 breast cancer cell line engineered to overexpress Zinc Finger Protein 703 (ZNF703). ZNF703 is a common Luminal B breast cancer oncogene that differentially regulates luminal and basal progenitors in human mammary epithelium. Manipulation of ZNF703 expression in the luminal MCF7 cell line modified the effects of TGF? on proliferation. ZNF703 drives the exprsesion of the green fluorescent protein ZsGreen. Please note there is a MCF7 Lenti-control cell line available to use as a negative control with this cell line (see Related tab).
  • Research area: Cancer; Cell signaling and signal transduction

  • For Research Use Only

Target Details

  • Target: Zinc Finger Protein 703 (ZNF703) (UniProt ID: Q9H7S9)

Application Details

  • Application notes: The pHIV-Zsgreen plasmid was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox-WPRE content of the pSICO plasmid (Ventura et al., 2004). ZsGreen is used as a reporter for ZNF703.

Handling

  • Format: Frozen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Holland et al. 2011. EMBO Mol Med. 3(3):167-80. PMID: 21337521.