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Contributor Information

  • Name Martin Clynes
  • Institute Dublin City University

Tool Details

  • Tool name: DLKP-I cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: DLKP
  • Organism: Human
  • Tissue: Lymph Node
  • Cancer type: Lung cancer
  • Disease: Cancer
  • Growth properties: monolayer
  • Description: Sub types of lung carcinoma with well-defined morphology and characteristic studies
  • Research area: Cancer; Drug development
  • Production details: Single cell cloning from DLKP parental population
  • Cellosaurus ID: CVCL_H738

  • For Research Use Only

Target Details

Application Details

  • Application notes: Intermediate sub-population cloned at NCTCC fromDLKPP5, about 25% ofDLKPshowDLKP-I morphology. Interconversion between DLKP-M/DLKP-SQ and DLKP-I, tetraploid, fold resistance compared to DLKP: 0.5 Adr; 0.65 Vcr; 0.6 VP-16. Immunocytochemistry: cytokeratin (all) -; GFAP-; vimentin+; neurofilament+; P-gp-; a2-integrin+; desmin-; 703D4+; 704A1+.

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F12, 5% FCS. Split 1:5, medium change 2-3 times weekly. Freezing method: 10% DMSO in FBS
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Keenan et al. 2012. Exp Cell Res. 318(5):593-602. PMID: 22281030.