CELL LINES
Contributor Information
- Name Richard Grose ; Gabriella Ficz ; Ateequllah Hayat
- Institute Queen Mary University of London
Tool Details
- Tool name: MCF10A-HER2
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A
- Tissue: Breast
- Cancer type: Breast cancer
- Disease: Cancer
- Model: Immortalised Line
- Conditional: Yes
- Conditional description: Conditional HER2 expression under tetracyline response element (TRE) regulated by the addition of doxycyline in breast epithelial cell line (MCF10A)
- Description: Expressed at low levels in normal breast tissues but overexpressed in a subset of breast and ovarian cancers. The GFP is in the same construct as the inducible HER2 but both genes are regulated by two different promoters. GFP expression is under the control of a weak EF1ÄÂ? promoter whereas the HER2 is induced by a strong TRE promoter.
- Research area: Cancer
- Production details: Inducible HER2 construct was stably transduced in MCF10A cell line using a third generation lentiviral transduction system.
- For Research Use Only
Target Details
- Target: Human Epidermal Receptor 2 (HER2/EBB2/neu), overexpressed in 25% breast cancers, treatments include Trastuzumab and Lapatinib. This plasmid also contains GFP for selection and tracking purposes.
Application Details
- Application notes: The GFP is in the same construct as the inducible HER2 but both genes are regulated by two different promoters. GFP expression is under the control of a weak EF1a promoter whereas the HER2 is induced by a strong TRE promoter.
Handling
- Format: Frozen
- Shipping conditions: Dry ice
Documentation
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