CELL LINES
Contributor Information
- Name Sarah A Martin
- Institute Queen Mary University of London
Tool Details
- Tool name: JU77R cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: JU77
- Organism: Human
- Tissue: Lung
- Cancer type: Lung
- Disease: Cancer
- Growth properties: Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20).
- Model: Cancer Model
- Research area: Cancer
- Production details: JU77 cells were incubated with 10ug/ml ADI-PEG20 in RPMI-1640 media supplemented with 2% FBS and re-treated every week. After approximately 2 months, colonies were picked and seeded in a 96-well plate with 10ug/ml ADI-PEG20. A surviving clone (JU777R) was gradually expanded and maintained in 1ug/ml ADI-PEG20.
- Cellosaurus ID: TBC
- For Research Use Only
Target Details
- Target: Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20)
Application Details
Handling
- Format: Frozen
- Growth medium: RPMI-1640 media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, and 100ug/ml streptomycin at 37Ă°C/5% CO2.
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Locke et al. 2016. Cell Rep. 16(6):1604-1613. PMID: 27452468.
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