Histone deacetylase 3 (HDAC 3) knockout mouse embryonic stem cell line
- Name Shaun Cowley
- Institute University of Leicester
Tool name: Histone deacetylase 3 (HDAC 3) knockout mouse embryonic stem cell line
Tool type: Cell Lines
Conditional description: Induction of Cre activity by addition of 4-hydroxy tamoxifen (OHT) to the growth media resulted in complete recombination of each allele and deletion of exon 2 (HDAC1ÄÂ?Â2/ÄÂ?Â2 or HDAC2 ÄÂ?Â2/ÄÂ?Â2) within 24 h
Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Histone deacetylase 3 (HDAC3) acts as the catalytic core of the SMRT/NCoR co-repressor complex which regulates chromatin structure and gene expression. It was recently shown that HDAC3 binds, and is regulated in vitro, by the binding of inositol phosphates (IP).
Research area: Developmental biology
Production details: E14 ES cells, expressing a CreER fusion protein from the ROSA26 locus, were used to generate HDAC3Lox/Lox; CreER cell lines by consecutive rounds of gene targeting.
- For Research Use Only
- • ANALYSIS OF THE PHYSIOLOGICAL ROLE OF HISTONE DEACETYLASE 3 (HDAC3) AND ITS REGULATION BY INOSITOL PHOSPHATES
- • Simandi et al. 2016. Mol Cell. 63(4):647-661. PMID: 27499297.