CELL LINES
Contributor Information
- Name Marielle Gold ; Erin Meerseier ; Irina Kurtz ; David Lewinsohn
- Institute Oregon Health & Science University (OHSU)
Tool Details
- Tool name: Immorta-MAIT T cell clone IK-C1 cell line
- Tool type: Cell Lines
- Organism: Human
- Disease: Mycobacterium tuberculosis
- Growth properties: TRAV1-2 is expressed uniformly (determined by flow cytometry staining). This clone expresses CD4, CD8, CD161, and CD27 and can be classified as a double-positive thymocyte. This T cell clone binds the MR1/5-OP-RU tetramer but not MR1/6FP tetramer (negative control). Clone is MR1-restricted in its production of IFN-gamma by ELISPOT test, determined by its response to M. smegmatis-infected A549 cell line but not a M. smegmatis-infected MR1-- A549 cell line.
- Model: Immortalised Line
- Conditional: No
- Description: Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in autoimmune diseases such as multiple sclerosis, rheumatiod arthritis, and systemic lupis erythematosus.
- Research area: Immunology
- Production details: Generated by single cell isolation of MR1/5-OP-RU tetramer+ cells from a human thymus. Single cells were rapidly expanded into a T cell clone using antibody to CD3 (clone OKT3) and IL-2.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: Can be use with conventional T cell expansion methods and proliferates under simple culture methods. Cell line can be maintained for at least 3 months.Cultures can be established by centrifugation with subsequent resuspension at 1 x 10^5 viable cells/mL in complete RPMI-1640 medium (10% heat-inavtivated FBS). Optional recombinant IL-2 at 1ng/mL. T-25 flask is recommended for culturing. Recommended concentration to maintain cultures between 1x10^5 and 1x10^6 viable cells/mL. Fresh medium recommended weekly, based on density and growth rate.
- Shipping conditions: Dry ice
Documentation
- Available on request
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