Product Image

Contributor Information

  • Name H. Grossman ; Anita Sabichi
  • Institute University of Michigan

Tool Details

  • Tool name: UM-UC-3 (Human bladder transitional cell carcinoma) cell line
  • Alternate names: UM-UC-3
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Bladder
  • Cancer type: Human bladder translational cell carcinoma
  • Disease: Cancer
  • Description: UM-UC-3 are a human transitional cell carcinoma of the bladder. These cells were assessed for their susceptibility to adenoviral mediated gene delivery, tumor growth in nude mice and differences in genetic alterations. Tumorigenicity studies in nude mice revealed the UM-UC-3 produced tumors, 1-1.5 cm in diameter in < 3 weeks. UM-UC-3 were more resistant to adenoviral gene transduction than several of the other cells evaluated. UM-UC-3 also had intermediate levels of Coxsackie adenovirus receptor (CAR) expression of the cells tested. These assays allowed for characterization of some of the most important features in each of the respective lines in an effort to more accurately establish commonly observed phenomena across cells of the same type of neoplasm.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Cells were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% heat inactivated Fetal Bovine Serum (FBS) and were supplemented with 1% penicillin and streptomycin at 37°C in 5% CO2 environment.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Sabichi et al. 2006. J Urol. 175(3 Pt 1):1133-7. PMID: 16469639.
  •   Grossman et al. 1984. J Urol. 132(4):834-7. PMID: 6471236.