CELL LINES
Contributor Information
- Name H. Grossman
- Institute University of Michigan
Tool Details
- Tool name: UM-RC-3 (Human renal carcinoma) cell line
- Tool type: Cell Lines
- Organism: Human
- Tissue: Bladder
- Cancer type: Genitourinary cancer
- Disease: Cancer
- Growth properties: Adherent
- Model: Cancer Model
- Conditional: No
- Description: UM-RC-3 are a renal carcinoma cell line, established in long term culture, from surgically obtained renal carcinoma specimens of patients who underwent nephrectomy. This patient had metastatic disease at the time of nephrectomy. Initial work suggests these cells grow well in primary culture but life expectancy is limited to less than 10 passages. These cells were evaluated for their ability to form tumors in nude mice and were confirmed to produced clear cell tumors. UM-RC-3 cells demonstrated no autologous reactivity in Protein A assays in low passage number cells but did in higher passage numbers. These cells are adherent and display a loss of contact inhibition. Doubling time is approximately 29 hours.
- Research area: Cancer
- For Research Use Only
Target Details
- Target: UM-RC-3
Application Details
Handling
- Format: Frozen
- Growth medium: Cells were grown in Eagle's Minimum Essential Medium (EMEM) containing 1% nonessential amino acids, 2mM glutamine, 100U/ml penicillin, 100ug/ml streptomycin and 10% Fetal Bovine Serum (FBS).
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Grossman et al. 1985. J Surg Oncol. 28(3):237-44. PMID: 4038766.
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