143B m.8993T>G isogenic mTUNE mT45 Cell Line
- Name Payam Gammage
- Institute Cancer Research UK, Glasgow: The Beatson Institute
Tool name: 143B m.8993T>G isogenic mTUNE mT45 Cell Line
Alternate names: mTUNE 45, mT45
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: 143B
Cancer type: Sarcoma
Model: Tumour line
Description: The cellular metabolic environemnt is influenced by relative levels of coexistant mutant and wild-type mitochondrial DNAs (heteorplasmy) in a range of diseases for which mitochondrial dysfunction is a feature. Disease phenotypes may be rescued by returning heteroplasmy towards wild-type levels.
To investigate this approach, the team led by Payam Gammage have devised a series of cell lines which stably express stable levels of mutatnt m.8993T>G mtDNA heteroplasmy.
Cell lines bearing m.8993T>G heteroplasmy of 80, 45, and 10% are available at CancerTools.org.
These lines are derivatives of the m.8993T cybrid cell developed by Prof Eric Schon, and are of of 143B human osteosarcoma lineage. This is part of a series of three cell line (mTUNE); see Related research tools tab.
Research area: Cancer; Metabolism
Production details: mTUNE cells were generated by Dr Michal MinczukÄË?Â?Âs lab and derive from female human osteosarcoma 143B (RRID: CVCL_2270) cybrid cells (Porteous et al., 1998), after correction of m.8993T>G mutation with mitochondrially-targeted zinc finger nucleases (Gammage et al., 2016a). See Gaude et al., 2018 for further details.
Additional notes: This is part of a series of three cell line (mTUNE); see Related Reagents tab.
- For Research Use Only
- • Gammage et al. 2018. Nat Med. 24(11):1691-1695. PMID: 30250142.
- • Gammage et al. 2018. Methods Mol Biol. 1867:43-56. PMID: 30155814.
- • Gaude et al. 2018. Mol Cell. 69(4):581-593.e7. PMID: 29452638.
- • Gammage et al. 2016. Methods Mol Biol. 1351:145-62. PMID: 26530680.
- • Gammage et al. 2016. Nucleic Acids Res. 44(16):7804-16. PMID: 27466392.
- • Gammage et al. 2014. EMBO Mol Med. 6(4):458-66. PMID: 24567072.