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Contributor Information

  • Name Payam Gammage
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: 143B m.8993T>G isogenic mTUNE mT10 Cell Line
  • Alternate names: mTUNE 1, mT1
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: 143B
  • Organism: Human
  • Tissue: Bone
  • Cancer type: Bone; Osteosarcoma
  • Disease: Cancer
  • Model: Tumour line
  • Description: The cellular metabolic environemnt is influenced by relative levels of coexistant mutant and wild-type mitochondrial DNAs (heteorplasmy) in a range of diseases for which mitochondrial dysfunction is a feature. Disease phenotypes may be rescued by returning heteroplasmy towards wild-type levels. To investigate this approach, the team led by Payam Gammage have devised a series of cell lines which stably express stable levels of mutatnt m.8993T>G mtDNA heteroplasmy. Cell lines bearing m.8993T>G heteroplasmy of 80, 45, and 10% are available at CancerTools.org. These lines are derivatives of the m.8993T cybrid cell developed by Prof Eric Schon, and are of of 143B human osteosarcoma lineage. This is part of a series of three cell line (mTUNE); see Related research tools tab.
  • Research area: Cancer; Metabolism
  • Production details: mTUNE cells were generated by Dr Michal Minczuk’s lab and derive from female human osteosarcoma 143B (RRID: CVCL_2270) cybrid cells (Porteous et al., 1998), after correction of m.8993T>G mutation with mitochondrially-targeted zinc finger nucleases (Gammage et al., 2016a). See Gaude et al., 2018 for further details.
  • Additional notes: This is part of a series of three cell line (mTUNE); see Related Reagents tab.

  • For Research Use Only

Target Details

  • Target: m.8993T>G

Application Details

Handling

  • Format: Frozen
  • Growth medium: Avoid unnecessary freeze/thaw cycles and keep passage duration to below 2-3 months to avoid heteroplasmy shifts.
  • Shipping conditions: Dry ice

Documentation

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  • Available on request

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References

  • •  Gammage et al. 2018. Nat Med. 24(11):1691-1695. PMID: 30250142.
  • •  Gammage et al. 2018. Methods Mol Biol. 1867:43-56. PMID: 30155814.
  • •  Gaude et al. 2018. Mol Cell. 69(4):581-593.e7. PMID: 29452638.
  • •  Gammage et al. 2016. Methods Mol Biol. 1351:145-62. PMID: 26530680.
  • •  Gammage et al. 2016. Nucleic Acids Res. 44(16):7804-16. PMID: 27466392.
  • •  Gammage et al. 2014. EMBO Mol Med. 6(4):458-66. PMID: 24567072.