IL-10-expressing Raw 264.7 cell line
- Name Jose Luis Pedraz ; JesĂşs Ciriza ; Laura Saenz del Burgo
- Institute University of the Basque Country, EHU
Tool name: IL-10-expressing Raw 264.7 cell line
Alternate names: IL-1, Interleukin 1
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: Raw 264.7
Cancer type: Epithelial to mesenchymal transition
Description: Microencapsulated macrophages release conditioned medium able to prevent epithelial to mesenchymal transition.
Epithelial to mesenchymal transition (EMT) has emerged as a key process in the development of renal fibrosis. In fact, EMT-derived fibroblasts contribute to the progression of chronic renal disease. In addition, anti-inflammatory M2 macrophages have exhibited a great influence on renal fibrosis. However, because of the high impact that the inputs of different environmental cytokines have on their phenotype, macrophages can easily lose this property. This is why we aimed to modify the Raw 264.7 cell line in order to induce a M2 stable phenotype.
M1 and M2 macrophages are main characters in the fibrotic process, chiefly preventing epithelial to mesenchymal transition (EMT). EMT, a process that make fully differentiated epithelial cells undergo transition to a fibroblast phenotype, has emerged as one important pathway in the development of renal fibrosis. Cells in EMT acquire mesenchymal migratory capacity and travel across the disrupted tubular basement membrane into the interstitial microenvironment. EMT-derived fibroblasts within the interstitium contribute to the progression of chronic kidney disease by facilitating deposition of interstitial extracellular matrix. The use of transplanted macrophages, pumping out active factors directly at the site, has proven to be an emergent technology in order to restore kidney function or modulate fibrosis through its role on EMT. However, macrophages, when transplanted in vivo, can lose their beneficial properties, depending on the environments. Thus, to preserve the macrophage regenerative effects, these cells were modified in order to induce and maintain their M2 phenotype.
Research area: Cancer ; Extracellular Matrix ; Stem Cell Biology
- For Research Use Only