CELL LINES
Contributor Information
- Name MIchal Hayun
- Institute Rambam Health Care Campus
Tool Details
- Tool name: MHYO-LCL1 Pre-leukaemic (DNMT3a R882H mutant) Human B-lymphoblast Cell Line
- Tool type: Cell Lines
- Tool sub-type: Primary
- Parental cell line: Novel patient-derived (male; acute myeloid leukaemia) cell line
- Organism: Human
- Tissue: Blood
- Cancer type: Blood cancer
- Disease: Cancer
- Growth properties: Confirmed antigen expression: CD19, CD20, CD27, CD45, HLA-DR, CD49d. Cytokine secretion: IL-10
- Model: Tumour line
- Conditional: No
- Description: THe MYHO-LCL cell line is very unique since it carries the pre-leukaemia DNMT3a R882H mutation, and therefore is useful in the investigation of B lymphocyte lineages. The cell line phenotype is of memory B lymphocytes, and therefore is relevant to B-cell development, oncology, and immunology. Relevant research areas include: Drug discovery, B-cell biology, B-cell development and transformation, aging, and acute myeloid leukaemia. These cells are monoclonal as confirmed by IGH gene clonality assay.
- Research area: Cancer
- Production details: This cell line was spontaneously grown from peripheral blood drawn from a male acute myeloid leukaemia patient upon diagnosis. This line was established in culture four weeks after seeding the AML blast cells. The cells contain low EBV mRNA expression and secret EBV into media, confirming immortalisation and transformation. These cells are in suspension and mostly grow as aggregates.
- For Research Use Only
Target Details
- Target: DNMT3a R882H mutant
Application Details
- Application notes: Relevant research areas include: Drug discovery, B-cell biology, B-cell development and transformation, aging, and acute myeloid leukaemia. These cells are monoclonal as confirmed by IGH gene clonality assay.
Handling
- Format: Frozen
- Growth medium: Cells have been grown in IScove Modified Dulbecco's Medium containing 10% foetal bovine serum and 1% pencicillin/streptomycin. Cells are grown at 37 degrees celsius and 5% CO2. Cultures were split at confluency 1:2 or 1:3 two or three times per week.
- Shipping conditions: Dry ice
Documentation
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