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Contributor Information

  • Name MIchal Hayun
  • Institute Rambam Health Care Campus

Tool Details

  • Tool name: MHYO-LCL1 Pre-leukaemic (DNMT3a R882H mutant) Human B-lymphoblast Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Primary
  • Parental cell line: Novel patient-derived (male; acute myeloid leukaemia) cell line
  • Organism: Human
  • Tissue: Blood
  • Cancer type: Pre-leukaemia, Leukaemia, Cancer,
  • Disease: Cancer
  • Description: THe MYHO-LCL cell line is very unique since it carries the pre-leukaemia DNMT3a R882H mutation, and therefore is useful in the investigation of B lymphocyte lineages. The cell line phenotype is of memory B lymphocytes, and therefore is relevant to B-cell development, oncology, and immunology.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Cells have been grown in IScove Modified Dulbecco's Medium containing 10% foetal bovine serum and 1% pencicillin/streptomycin. Cells are grown at 37 degrees celsius and 5% CO2. Cultures were split at confluency 1:2 or 1:3 two or three times per week.
  • Shipping conditions: Dry ice

Documentation

  • Available on request