CELL LINES
Contributor Information
- Name Michal Hayun
- Institute Rambam Health Care Campus
Tool Details
- Tool name: MHYO-LCL2 Human B-lymphoblast Cancer Cell Line
- Tool type: Cell Lines
- Tool sub-type: Primary
- Parental cell line: Novel patient-derived (female; acute myeloid leukaemia) cell line
- Organism: Human
- Tissue: Blood
- Cancer type: Leukaemia; Cancer; AML
- Disease: Cancer
- Growth properties: B-lymphoblast. Morphology: B-lymphoblastoid. Confirmed antigen expression: CD19, CD20, CD27, CD38, IgG. Confirmed cytokine secretion: IL-6, IL-10.
- Model: Tumour line
- Conditional: No
- Description: The MHYO-LCL2 is an immortalised cancer cell line derived from a patient with acute myeloid leukaemia. The cell type is B-lymphoblast. This cell line is a useful tool for reactivation of EBV-specific cytotoxic T-lymphocytes in vitro. Relevant research areas include: Drug discovery, B-cell biology, B-cell development and transformation, and acute myeloid leukaemia. These cells are polyclonal as confirmed by IGH gene clonality assay.
- Research area: Cancer; Drug discovery; B-cell biology; B-cell development; transformation
- Production details: This cell line was spontaneously grown from peripheral blood drawn from a female acute myeloid leukaemia patient upon diagnosis. This line was established in culture four weeks after seeding the AML blast cells. The cells contain low EBV mRNA expression and secret EBV into media, confirming immortalisation and transformation. These cells are in suspension and mostly grow as aggregates.
- For Research Use Only
Target Details
- Target: Leukaemia
Application Details
- Application notes: These cells are polyclonal as confirmed by IGH gene clonality assay.
Handling
- Format: Frozen
- Growth medium: Cells have been grown in IScove Modified Dulbecco's Medium containing 10% foetal bovine serum and 1% pencicillin/streptomycin. Cells are grown at 37 degrees celsius and 5% CO2. Cultures were split at confluency 1:2 or 1:3 two or three times per week.
- Shipping conditions: Dry ice
Documentation
- Available on request
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