CELL LINES
Contributor Information
- Name Carmel McConville
- Institute University of Birmingham
Tool Details
- Tool name: BCH-RB53 Retinoblastoma cell line
- Tool type: Cell Lines
- Tool sub-type: Primary
- Organism: Human
- Tissue: Stromal
- Gender: Female
- Cancer type: Eye cancer
- Disease: Cancer
- Model: No BCOR mutation present
- Description: Retinoblastoma is a rare type of eye cancer most commonly affecting children under the age of 5. One eye can be affected, which is called unilateral retinoblastoma. Bilateral retinoblastoma is when both eyes are affected. This can be very distressing and frightening for the child and their parents. Some children are born with a change mutation in the RB1 retinoblastoma gene inherited from one of their parents. Or this gene change happened during the very early stages of their development in the womb. About 4 out of 10 children diagnosed (about 40%) have this heritable type, which often affects both eyes. Retinoblastoma tends to develop in just one eye in children who do not have this heritable type. We don't know what causes the remaining 60% of retinoblastomas. But these non inherited types nearly always only affect one eye. https://www.cancerresearchuk.org/about-cancer/childrens-cancer/eye-cancer-retinoblastoma/about
- Research area: Cancer
- Cellosaurus ID: TBC
- Additional notes: STR data available on request
- For Research Use Only
Target Details
Application Details
- Application notes: Tissue used: Stromal Patient Sex: Female No BCOR mutation present STR data available on request
Handling
- Format: Frozen
- Growth medium: Culture conditions: DME/F12 + 15% fetal calf serum + glutamine + non-essential amino acids + beta-mercaptoethanol - dilute fresh stock of b-ME and add just before use (Carmel needs to check concentrations). Cell line is very slow growing and should only be split at high density (e.g. 2x 25cm flasks -> 3 flasks). Before splitting, shake flask gently to dislodge apoptotic cells from surface of clumps, allow clumps to settle, remove medium/cell debris and replace with fresh medium. Do not completely disaggregate the cell clumps as this will reduce cell viability.Growth pattern: in clumps in suspension Most of these cell lines are very slow growing and should only be split at high density e.g. 2 x 25cm flasks -> 3 flasks. Before splitting, shake flask gently to dislodge apoptotic cells from surface of clumps, allow clumps to settle, remove medium/cell debris and replace with fresh medium. Do not completely disaggregate the cell clumps as this will reduce cell viability.
- Shipping conditions: Dry ice
Documentation
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