CELL LINES
Contributor Information
- Name Alessandra Cambi
- Institute Radboud University Medical Center
Tool Details
- Tool name: CHO DC-SIGN-N80A cell line
- Alternate names: DC-SIGN N8A
- Tool type: Cell Lines
- Parental cell line: CHO
- Organism: Hamster
- Tissue: Ovary
- Disease: HIV-1; Ebola Virus; Hepatitis C virus
- Conditional: No
- Description: Dendritic Cell-Specific ICAM-3-Grabbing Non-Integrin (DC-SIGN; CD209) is a type II plasma membrane PRR abundantly expressed in antigen-presenting cells such as dendritic cells (DCs) and activated macrophages. As member of the C-type Lectin Receptor (CLR) family, DC-SIGN binds a plethora of pathogens, ranging from viruses like HIV-1, ebola virus, and hepatitis C virus, to larger pathogens like Mycobacterium tuberculosis and Candida albicans. This N80A mutant lacks the N-glycosylation ligand binding domain.
- Research area: Cell Signaling & Signal Transduction; Immunology
- Production details: CHO cell lines stably expressing DC-SIGN-N80 were established by Lipofectamin 2000 transfection, and were cultured in HamâÂÂs F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5âÂÂmg/ml of the aminoglycoside antibiotic G418.
- For Research Use Only
Target Details
- Target: Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin lacking the ligand binding domain N80.
Application Details
Handling
- Format: Frozen
- Growth medium: HamâÂÂs F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5âÂÂmg/ml of the aminoglycoside antibiotic G418.
- Shipping conditions: Dry ice
Documentation
- Available on request
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