CELL LINES
Contributor Information
- Name Sergio F de Almeida
- Institute Instituto de Medicina Molecular JoĂÂo Lobo Antunes
Tool Details
- Tool name: Caki-2 DNMT3A KO cell line
- Alternate names: TBRS, HESJAS
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: Clear cell renal cell carcinoma cell line (Caki-2)
- Organism: Human
- Tissue: Kidney
- Model: Knock-Out
- Conditional: No
- Description: Experimental ablation of DNMT3A gene expression using the CRISPR/Cas9 system renders Caki-2-DNMT3A knockout cells a valuable tool to investigate the role of DNA methylation in processes such as cell differentiation or cancer progression.
- Research area: Cancer; DNA Damage and Repair; Epigenetics & Nuclear Signalling; Gene Expression
- Production details: CRISPR/Cas9 KO
- Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.
- For Research Use Only
Target Details
- Target: DNA methyltransferase 3A
Application Details
- Application notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license
Handling
- Format: Frozen
- Growth medium: Grow as monolayers in Dulbecco's modified Eagle medium, supplemented with 10% (v/v) FBS, 1% (v/v) nonessential amino acids, 1% (v/v) L-glutamine and 100U/mL penicillin-streptomycin, at 37Ă°C in a humidified atmosphere with 5% CO2.
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.
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