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Contributor Information

  • Name Sergio F de Almeida
  • Institute Instituto de Medicina Molecular JoĂŁo Lobo Antunes

Tool Details

  • Tool name: Caki-2 DNMT3A KO cell line
  • Alternate names: TBRS, HESJAS
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Clear cell renal cell carcinoma cell line (Caki-2)
  • Organism: Human
  • Tissue: Kidney
  • Description: Experimental ablation of DNMT3A gene expression using the CRISPR/Cas9 system renders Caki-2-DNMT3A knockout cells a valuable tool to investigate the role of DNA methylation in processes such as cell differentiation or cancer progression.
  • Research area: Cancer ; DNA Damage and Repair ; Epigenetics & Nuclear Signalling ; Gene Expression
  • Additional notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Grow as monolayers in Dulbecco's modified Eagle medium, supplemented with 10% (v/v) FBS, 1% (v/v) nonessential amino acids, 1% (v/v) L-glutamine and 100U/mL penicillin-streptomycin, at 37°C in a humidified atmosphere with 5% CO2.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.