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Contributor Information

  • Name Sergio F de Almeida
  • Institute Instituto de Medicina Molecular JoĂŁo Lobo Antunes

Tool Details

  • Tool name: Caki-2 SETD2 KO cell line
  • Alternate names: HBP231, HIF-1, HIP-1, HYPB, KMT3A
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Clear cell renal cell carcinoma cell line (Caki-2)
  • Organism: Human
  • Tissue: Kidney
  • Description: Experimental ablation of SETD2 gene expression using the CRISPR/Cas9 system renders Caki-2-SETD2 knockout cells a valuable tool to investigate the role of H3K36me3 histone methylation in processes such as transcription or DNA repair.
  • Research area: Cancer ; DNA Damage and Repair ; Epigenetics & Nuclear Signalling ; Gene Expression
  • Additional notes: Cancer Research Technology Limited (trading research tools as has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: This license from ERS Genomics Ltd allows to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Growth medium: Grow as monolayers in Dulbecco's modified Eagle medium, supplemented with 10% (v/v) FBS, 1% (v/v) nonessential amino acids, 1% (v/v) L-glutamine and 100U/mL penicillin-streptomycin, at 37°C in a humidified atmosphere with 5% CO2.
  • Shipping conditions: Dry ice


  • Available on request


  •   de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.